Para – aminoblebbistatin
Easily soluble, non-fluorescent, photostable myosin inhibitor
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2. Confirm your shipping&billing address via email
3. Receive your compound within 5-7 business days via FedEx
4. Pay via bank transfer or Credit Card
Price:
249 EUR/1 mg
996 EUR/5 mg
| Name of chemical: | Para-aminoblebbistatin |
| Other names: | P-amino-blebbistatin |
| para-amino-blebbistatin | |
| Chemical name: | (S)-1-(4-Amino-phenyl)-3a-hydroxy-6-methyl-1,2,3,3a-tetrahydropyrrolo[2,3-b]quinolin-4-one |
| Cat. No: | DR-Am-89 |
| Molecular weight | 307 |
| Extinction coeff in water at 427 nm: | 6860 M-1cm-1 (pH 7.3) |
| Extinction coeff in DMSO at 450 nm: | 9900 M-1cm-1 |
| Chemical purity: | >95% |
| Ratio of (-) enantiomer: | >90% |
| Storage conditions: | in powder form or dissolved in DMSO in aliquots at -20 oC to limit freezing-thawing cycles. |
| Solubility: | The equilibrium concentration of paraaminoblebbistatin is 300-420 uM in water based buffers containing 0.1%-1% DMSO. For the concentration of DMSO stocks, we recommend 50 mM. |
| Photostability: | Unlike blebbistatin, para-aminoblebbistatin is not phototoxic when irradiated with >440 nm wavelength light. |
| Myosin inhibition: | Myosin type inhibitory properties Rabbit skeletal muscle myosin S1 basal ATPase activity 100% inhibition IC50: ~1.3 µM Rabbit skeletal muscle myosin S1 actin activated ATPase activity 100% inhibition IC50: ~0.47 µM Dictyostelium discoideum myosin II motor domain actin activated ATPase activity 80% inhibition IC50 ~6.6 µM Dictyostelium discoideum myosin II motor domain basal ATPase activity 90 % inhibition IC50: ~6.7 µM Human, slow-twitch muscle fiber (beta myosin) (data kindly provided by Alfredo López) >95% inhibition IC50: ~11 µM pig left ventricle, isolated cardiac myosin ATPase acivity (unpublished data) >92% inhibition IC50: 5.2 µM |
| Cytotoxicity: | Unlike blebbistatin, para-aminoblebbistatin is not cytotoxic in the dark. |
| Alternative: | If para-aminoblebbistatin inhibitory properties are not ideal for your setup, para- nitroblebbistatin may be a useful alternative. (Reference: Para-nitroblebbistatin, the non- cytotoxic and photostable myosin II inhibitor by Miklós Képiró, Boglárka H. Várkuti, László Végner, Gergely Vörös, György Hegyi, Máté Varga, András Málnási-Csizmadia in Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8211-5. doi:10.1002/anie.201403540) |
